Ahmed Alsaid Othman, Rabab Mubarak And Marwa Sameer Moussa,

ABSTRACT
Background: Stem Cells From Human Exfoliated Deciduous Teeth (SHED) Are Considered One Of The Simplest; The Most Convenient Source Of Adult Stem Cells. The Study Aimed To Examine The Proliferative Effect Of Fibroblast Growth Factor 6 (FGF-6) On SHED And On CDK2 And MATEK Derived Microvesicles Genes Expression. Methods: SHED Was Cultured From Deciduous Teeth Pulp. SHED Were Divided Into Two Groups; Control Group And Test Group Without And With FGF-6 Supplementation Respectively. At The Third Passage, SHED Proliferation Was Assessed By MTT Assay. MVs Were Purified And CDK2 And MATK Genes Expression Were Assessed By Real Time Polymerase Chain Reaction.

Results: SHED Were Identified By Their Positivity For CD90 And CD73 And Negativity For CD 45 And CD 34. SHED Proliferation In The Test Group Was Significant In Comparison To Control Group (P < 0.001). SHED Derived MVs? MRNA From The Test Group Demonstrated A Remarkable Elevated Expression Of CDK2 And MAKT, (P< 0.002) (P< 0.005) Respectively In Comparison With Those Of The Control Group. Conclusion: FGF-6 Enhances Proliferation Of SHED. It Is Favorable For The Production Of Large Number Of Stem Cells Which Is Beneficial To Cell Based Therapies. SHED Derived MVs CDK2 And MAKT Can Be Used As Molecular Biomarkers For SHED Proliferation.